CHO-FCGR2B is a recombinant clonal stable CHO cell line expressing full length human FCGR2B (IgG Fc receptor II-b). Surface expression of FCGR2B was confirmed by flow cytometry using specific antibodies. Detailed information and characterisation can be found in the Product Sheet.
Target Aliases
Low affinity immunoglobulin gamma Fc region receptor II, CD32, FcyRIIb
Target Expression Level(s) Available
High (ca. 200000 molecules/cell as analysed by Quantibrite)
Cell Background
CHO-K1 (Chinese Hamster Ovary), epithelial-like cells, adherent but can be transferred into suspension.
Buying and Ordering the Cells
Academic Research
Non-profit academic and research institutions, universities, and governmental entities may purchase the cells for internal, non-commercial research under inscreenex’s Academic Research Limited Use Label License. This license is intended for internal research only.
Industry Research
Industry customers may purchase the cells for internal research and development under inscreenex’s Industry Research Limited Use Label License. This license is intended for internal R&D, drug discovery and assay development use only.
Commercial Use
Industry customers planning to use the cells for true commercial purposes require a separate Commercial Use license from inscreenex. This includes, for example, providing services to third parties, incorporating the cells into a product or platform, or using the cells in manufacturing, QC, or batch-/lot-release testing.
How to Order
To request a quote, please email us at or use the contact form provided below.
We are already registered on several digital order processing platforms, if you would like to add us as a registered supplier, please contact us at and we are happy to help.
Supplied as
Each license includes 2 vials of >2Mio viable cells. Additional or replacement vials can be purchased for a fee of 450€.
Target Info
Background
Fc gamma receptor IIb (FCGR2b) is a low-affinity receptor for the Fc region of complexed or aggregated immunoglobulin gamma. It participates in phagocytosis of immune complexes and modulates antibody production by B cells, including down-modulation of prior activation triggered via B-cell antigen receptors, T-cell antigen receptors, or another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis, and isoform IIB2 does not trigger phagocytosis. Several transcript variants encoding different isoforms are reported.
Variation in FCGR2B may increase susceptibility to systemic lupus erythematosus. In follicular lymphoma, chromosomal translocation t(1;22)(q22;q11) can deregulate FCGR2B, leading to hyperexpression, and this dysregulation may contribute to tumor progression.
Field of Research
- Immunology
- B-cell regulation
- Autoimmune disease
Therapeutics and Binding Partners
Selected Binding Partners
- Immunoglobulin heavy constant gamma 1 (IGHG1)
- Immunoglobulin heavy constant gamma 3 (IGHG3)
- Immunoglobulin heavy constant gamma 4 (IGHG4)
Product Details
Stable recombinant CHO cells at low and high confluency.
Expression of human FCGR2B in CHO-FCGR2B cells (INS-SF-1049) analyzed by Flow Cytometry.
Primary antibody: PE anti-human CD32B/C Antibody, Clone 4F5/CD32 (BioLegend #398403)
Flow Cytometry was performed according to the protocol provided under the “Protocols” tab.
Quantification: approx. 200000 molecules per cell (High) analyzed by BD QuantiBrite quantification kit (cat.no. 340495) according to the protocol provided under the “Protocols” tab.
We recommend the following reagents for the cell culture of this cell line:
Growth Medium: CHO Growth Medium D (INS-ME-1048)
Freezing Medium: Cell Freezing Medium (INS-SU-1027)
Coating: not required
Please note that compositions for the above listed reagents are available on their respective product sites, as well as in the cell line Product Sheets, should you wish to prepare your own reagents.
Coming Soon!
Stay tuned for comprehensive protocols for this cell line, from routine cell cultures to assay manuals.
Coming Soon!
Coming Soon!
Material:
- PBS/EDTA solution
- 2% FBS/FCS in PBS (FACS Buffer)
- primary antibody (concentration is indicated under “Target expression” tab)
Protocol:
Wash Protocol: Add FACS Buffer, resuspend cells gently, then centrifuge at 300×g for 5min.
- Prepare detection reagents in FACS buffer using the concentrations indicated under Materials.
- Aspirate medium from cells.
- Add PBS/EDTA solution to the cells and incubate at room temperature or 37°C for 5-10min, or until the cells detach.
- Wash cells 1×.
- Add primary antibody in FACS buffer, resuspend cells gently.
- Incubate at ambient temperature for 20-30min.
- Wash cells 2×.
- Resuspend in 100-200µl FACS Buffer.
- Analyse cells using a flow cytometer.
For a more detailed protocol, please see the BD Biosciences Quantibrite instructions.
Important: All instrument settings for fluorescence and compensation must be the same as the cellular assay settings.
- Reconstitute the BD Quantibrite beads using 0.5 mL of buffer, such as phosphate buffered saline (PBS) with sodium azide plus 0.5% bovine serum albumin (BSA), and vortex.
- Measure fluorescence of singlet beads using a flow cytometer and extract the geometric means of all four fluorescence peaks.
- Measure cells using the same instrument settings.
- Use the lot-specific values for the PE molecules per bead and linear regression to calculate the PE molecules per cell (approximation of target molecules per cell)
- For an even more meaningful result, contact the antibody provider for information on the number of fluorescent molecules per antibody.
Related Products
Product FAQ
Yes, for selected targets, we provide cell lines that exhibit varying expression levels. Detailed information regarding the availability of different expression levels can be found in both the ‘Product Summary’ and ‘Product Details’ sections.
Cell lines with available expression level variations are denoted by an abbreviation following the catalog number. For instance, ‘INS-SF-1020-MH’ denotes a cell line with medium-high expression levels, whereas ‘INS-SF-1020-L’ indicates a cell line with low expression of the same target.
Should you require a specific expression level not readily available in our standard product range, we also offer custom cell line generation services utilizing our advanced Landing Pad technology.
For several targets, we already have ready-to-use cell lines available in both CHO and HEK293 backgrounds. Please refer to Related Products section, or have a look at our product catalog to see if your target and the desired cell line are listed.
For any other specific cell line background such as A549, MIA PaCa-2, or any other cell line, we currently do not offer pre-established cell lines. However, we are pleased to provide a custom cell line development service. Through this service, we can express your target in virtually any cell background utilizing random integration techniques, tailored to meet your research needs.
For the best performance of this cell line, we advise using our Medium and Coating Solutions specified in the product sheet. These have been specially optimized for these cells.
All our stable recombinant cell lines are provided as adherent cultures, if not indicated otherwise in the Product Sheet.
Some of our CHO cell lines can be easily adapted to suspension culture. If a particular cell line can be cultured in suspension is indicated in the product summary section at the top of the page under Cell Background, as well as in the Product Sheet, where you will also find detailed protocols for suspension culture.
Our stable recombinant cell lines are generated using our proprietary Landing Pad Technology, in which we use targeted integration at pre-validated genomic locations to express the target. You can find more details on our Landing Pad technologies under our Landing Pad Services.
After cell line generation, target expression levels were validated by Flow Cytometry using the reagents and protocols indicated in the section Product Details or in the Product Sheet.
This cell line is BSL 1 according to German legislation. However, persons working with such cell lines and their employers are required to familiarize themselves with the national regulations and safety precautions, as they may differ. Our provided biosafety classification does not provide any exemption from this responsibility.
Literature and Reference
We love to hear about your research! Please let us know if you have published using our cells.