This Nav1.8 cell line is a recombinant clonal stable CHO cell line expressing full length human Sodium channel protein type 10 subunit alpha (SCN10A aka Nav1.8) plus beta subunit 1 (SCN1B). Electrophysiological characterization was performed by Metrion Biosciences. Detailed information and characterisation can be found in the Product Sheet.
Target Aliases
SCN10A, PN3, HPN3, SNS, Nav1.8
Cell Background
CHO-K1 (Chinese Hamster Ovary), epithelial-like cells, adherent but can be transferred into suspension.
- Cells validated by
Buying and Ordering the Cells
Academic Research
Non-profit academic and research institutions, universities, and governmental entities may purchase the cells for internal, non-commercial research under inscreenex’s Academic Research Limited Use Label License. This license is intended for internal research only.
Industry Research
Industry customers may purchase the cells for internal research and development under inscreenex’s Industry Research Limited Use Label License. This license is intended for internal R&D, drug discovery and assay development use only.
Commercial Use
Industry customers planning to use the cells for true commercial purposes require a separate Commercial Use license from inscreenex. This includes, for example, providing services to third parties, incorporating the cells into a product or platform, or using the cells in manufacturing, QC, or batch-/lot-release testing.
How to Order
To request a quote, please email us at or use the contact form provided below.
We are already registered on several digital order processing platforms, if you would like to add us as a registered supplier, please contact us at and we are happy to help.
Supplied as
Each license includes 2 vials of >2Mio viable cells. Additional or replacement vials can be purchased for a fee of 450€.
Target Info
Background
Nav1.8 (SCN10A) is a voltage-gated sodium channel predominantly expressed in peripheral sensory neurons, playing a crucial role in nociception and pain signaling. Its selective expression and involvement in generating and propagating action potentials in nociceptors make it a validated therapeutic target for chronic pain conditions, including neuropathic and inflammatory pain. Dysfunctional Nav1.8 activity is implicated in multiple pain-associated disorders, underscoring its significance in pharmaceutical research. Notably, Nav1.8-specific inhibitors, such as suzetrigine (VX-548) developed by Vertex, have shown clinical promise in providing analgesia with improved tolerability profiles. Recombinant Nav1.8-expressing CHO cell lines facilitate the high-throughput screening and identification of selective modulators, accelerating drug discovery and optimization processes aimed at treating pain-related diseases.
Field of Research
- Pain
- Neuropathy
- Non-opioid analgesics
Product Details
Stable recombinant CHO cells at low and high confluency, cultured in CHO Growth Medium A (INS-ME-1039) as a 2D monolayer.
Extensive electrophysiological validation by Metrion Biosciences using manual patch clamp and the QPatch and Qube automated patch clamp platform. See the Product Sheet for more details. Data below recorded on the QPatch.
| Parameter | Nav1.8 CHO Cell Line |
|---|---|
| Expressed Subunits | SCN10A (α) + SCN1B (β) |
| Expression | Constitutive (CMV) |
| Patch Clamp Platforms | Manual, QPatch, Qube |
| TTX Resistance | Yes (validated in presence of TTX) |
| Peak Current Amplitude | –0.87 nA |
| Activation V₀.₅ | 11.3 mV |
| Inactivation V₀.₅ | 49.4 mV (QPatch) |
| Seal Formation Success | 94% |
| Recording Success | 86% |
| Stability Over Time | Detectable through ≥65 passages |
| Reference Compounds IC₅₀ values (resting/inactivated) | VX-150: 158 nM / >1 µM PF-04531083: >3 µM / 355 nM |
We recommend the following reagents for the cell culture of this cell line:
Growth Medium: CHO Growth Medium A (INS-ME-1039)
Freezing Medium: Cell Freezing Medium (INS-SU-1027)
Coating: not required
- Product Sheet including all relevant protocols and instructions (PDF)
- Material Safety Data Sheet (PDF)
- Request CoA (Email)
Coming Soon!
Stay tuned for comprehensive protocols for this cell line, from routine cell cultures to assay manuals.
Coming Soon!
Coming Soon!
Related Products
Product FAQ
What cell background is used for these models?
Both the Nav1.7 and Nav1.8 cell lines are based on a proprietary CHO-K1-derived landing pad cell line developed by inscreenex. This platform allows site-specific, single-copy integration and supports stable, high-level expression in both adherent and suspension formats.
Are the cells compatible with automated patch clamp systems?
Yes. The Nav1.7 and Nav1.8 cell lines were validated on the Sophion QPatch platform, and Nav1.8 was additionally tested on the Qube system. Both cell lines demonstrated high seal formation and recording success rates, confirming suitability for automated workflows.
What electrophysiological data are included?
Each cell line is accompanied by a comprehensive data package including:
Peak current amplitudes
Activation and inactivation V₀.₅ values
Pharmacological IC₅₀ values for reference compounds
Recording success rates on automated systems
All data were generated by Metrion Biosciences Ltd, a leading CRO in ion channel electrophysiology.
Can CROs use these cells to provide services?
es. The Limited Commercial Use License (LCUL) permits CROs to use the cells:
Under contract with a licensed client, or
Through direct licensing to offer fee-for-service work to third parties
Do you offer customized versions of these cell lines?
Yes. inscreenex offers:
Gain- and loss-of-function mutants for Nav1.7 and Nav1.8 (e.g., IEM, PEPD, CIP)
Species-specific orthologs (mouse, rat, dog, Cynomolgus monkey)
All custom models are built using the same CHO landing pad platform.
What is included with the order?
Each order includes:
Two cryopreserved vials of the selected cell line (additional vials available for a service fee)
Detailed protocols for thawing, freezing, culture (adherent and suspension)
Patch clamp solution compositions and voltage protocols
Are the cells in stock and how fast is shipping?
Yes, both Nav1.7 and Nav1.8 cell lines are in stock and usually ship within 1–2 business days.
Estimated shipping times:
EU: 1–2 business days
UK: 2–3 business days
USA: 3–4 business days
Rest of world: please contact us for estimated delivery timelines
What is the BSL of the stable cell lines?
The cell lines are BSL1.
Can I use the cells commercially?
Yes, industry customers planning to use the cells for true commercial purposes may request a separate Commercial Use license from inscreenex. This includes, for example, providing services to third parties, incorporating the cells into a product or platform, or using the cells in manufacturing, QC, or release workflows.
Industry customers planning to use the cells for internal R&D, drug discovery and assay development may purchase the cells under the inscreenex Industry Research Limited Use Label License.
How do I order the cell lines?
What is the pricing model?
The cells are available under a one-time access fee, with no royalties or ongoing costs. Pricing includes two vials and the full licensing and protocol package. A discounted introductory offer is available when both cell lines are purchased together.
Literature and Reference
We love to hear about your research! Please let us know if you have published using our cells.