Proprietary mix of extracellular matrix (ECM) proteins that is optimized for the culture of our immortalized cells and comes as a ready-to-use solution.
This Coating Solution directly replaces the following products, which are now discontinued: huAEC Coating Solution (INS-SU-1018), Epithelial Coating Solution (INS-SU-1020) and Endothelial Cell Coating Solution (INS-SU-1022). For more information see the FAQ below.
Characteristics
- Ready-to-use solution
- Blend of ECM proteins
- Coating solution
Contents
- 50, 100 or 300ml ready-to-use solution
Cell Lines
- CI-hAELVi (INS-CI-1015)
- CI-huArlo (INS-CI-1031)
- CI-huBroBEC (INS-CI-1025)
- CI-huThyrEC (INS-CI-1017)
- CI-huEGC (INS-CI-1030)
- CI-muADINTESTI (INS-CI-1018)
- CI-pNaEC (INS-CI-1029)
- CI-huVEC (INS-CI-1002)
- CI-huMEC (INS-CI-1008)
- CI-muMEC (INS-CI-1004)
Buying and Ordering
To request a quote, please email us at or use the contact form provided below.
We are already registered on several digital order processing platforms, if you would like to add us as a registered supplier, please contact us at and we are happy to help.
Product Details
Coating Solution is a specially developed coating solution for optimal attachment and growth of our immortalized cells:
- CI-hAELVi (INS-CI-1015)
- CI-huArlo (INS-CI-1031)
- CI-huBroBEC (INS-CI-1025)
- CI-huThyrEC (INS-CI-1017)
- CI-huEGC (INS-CI-1030)
- CI-muADINTESTI (INS-CI-1018)
- CI-pNaEC (INS-CI-1029)
- CI-huVEC (INS-CI-1002)
- CI-huMEC (INS-CI-1008)
- CI-muMEC (INS-CI-1004)
.
Epithelial Coating Solution is provided as a ready-to-use solution.
It contains a proprietary mix of extracellular matrix (ECM) proteins that have been found to optimally support the attachment and growth of our epithelial and various other cell lines.
Store the products as follows if not indicated otherwise on the product label.
Coating Solution: at 4 to 8°C
Coated cell culture vessels: sealed at 4 to 8°C
Please see the product label for the expiry date of your product.
To prepare coated cell culture surfaces:
- Cover the cell culture vessel with Coating (see info below for recommended volumes). Rock back and forth to cover entire surface if necessary.
- Incubate for at least 2h at 37°C in the incubator or overnight at 2–8°C.
- Aspirate huAEC Coating.
- Wash once with at least 4 volumes of sterile PBS.
- Aspirate PBS.
Use immediately or store coated cell culture plastic according to instructions provided under „Storage“.
Flask/Plate | Coating Volume
T75 | 3ml
T25 | 1ml
6-well | 0.7ml
12-well | 0.25ml
24-well | 0.1ml
48-well | 75µl
96-well | 50µl
Instructions for storage and coating
Material Safety Data Sheet (MSDS)
Related Products
Product FAQ
huAEC Coating (INS-SU-1018), Epithelial Coating (INS-SU-1020), Endothelial Coating (INS-SU-1022) and this new Coating Solution (INS-SU-1026) share the exact same composition and pricing. There is no change to usage protocols, you may continue to use the new Coating Solution in exactly the same way as the three products listed above.
We have streamlined our product portfolio by consolidating the three coating solutions into a single product under a single name. This simplifies ordering and cell culture protocols.
Yes, this reagent is provided as a sterile, ready-to-use solution.
This is a proprietary blend of extracellular matrix proteins optimized for cell attachment and growth. We do not disclose the exact composition of this Coating Solution. However, if you have specific components that might interfere with any of your assays, we are happy to help you. Just reach out to us!
Depending on the product, we may be able to offer custom formulations, for example excluding specific supplements from the medium. Please contact us and we will work with you to find the best solution.