Collagen is the most widely used extracellular matrix protein for cell culture, facilitating cell attachment, growth and differentiation. Our Collagen Coating Solutions is optimized for the culture of our immortalized cells. It comes as a ready-to-use, sterile solution and is based on Collagen Type-1 derived from rat tails.
Characteristics
- Ready-to-use solution
- Collagen Type-I, derived from rat tails
- 0.1mg/ml (0.01%) in water
Contents
- 50, 100 or 300ml ready-to-use solution
Cell Lines
- CI-muINTEPI (INS-CI-1007)
- CI-muHepa (INS-CI-1012)
- CI-huFIB – only in serum-free culture system (INS-CI-1010)
Buying and Ordering
To request a quote, please email us at or use the contact form provided below.
We are already registered on several digital order processing platforms, if you would like to add us as a registered supplier, please contact us at and we are happy to help.
Related Products
Product Details
Collagen Coating Solution is a specially developed Type-Icollagen coating solution for optimal attachment and growth of various immortalized cells CI-muINTEPI (INS-CI-1007), CI-muHepa (INS-CI-1012), CI-huFIB – only in serum-free culture system (INS-CI-1010).
Collagen Coating Solution is provided as a ready-to-use solution.
Collagen coating solution enhances in vitro cell culture by promoting cell adhesion, proliferation, and differentiation. It mimics the extracellular matrix, providing a more physiologically relevant environment for various cell types, thereby improving the reliability and applicability of experimental results in research and tissue engineering.
Store the products as follows if not indicated otherwise on the product label.
Coating Solution: at 4 to 8°C
Coated cell culture vessels: sealed at 4 to 8°C
Please see the product label for the expiry date of your product.
To prepare coated cell culture surfaces:
- Cover the cell culture vessel with huAEC Coating (see info below for recommended volumes). Rock back and forth to cover entire surface if necessary.
- Incubate for at least 2h at 37°C in the incubator or overnight at 2–8°C.
- Aspirate huAEC Coating.
- Wash once with at least 4 volumes of sterile PBS.
- Aspirate PBS.
Use immediately or store coated cell culture plastic according to instructions provided under „Storage“.
Flask/Plate | Coating Volume
T75 | 3ml
T25 | 1ml
6-well | 0.7ml
12-well | 0.25ml
24-well | 0.1ml
48-well | 75µl
96-well | 50µl
Instructions for storage and coating
Material Safety Data Sheet (MSDS)
Product FAQ
Yes, this reagent is provided as a sterile, ready-to-use solution.
This coating solution is composed of 0.1mg/ml (0.01%) Collagen in water. The collagen used is Type-I collagen derived from rat tails.
Depending on the product, we may be able to offer custom formulations, for example excluding specific supplements from the medium. Please contact us and we will work with you to find the best solution.