Freezing Medium

Freezing Medium is a specially developed cryopreservation medium freezing of our immortalized cells.

This is a proprietary mix of serum and DMSO, optimized for high viability and survival of our immortalized cells.

This Medium is provided as a ready-to-use product.

Store the products as follows if not indicated otherwise on the product label.

Freezing Medium -18 to -20°C, avoid repeated freeze-thaw cycles. Aliquot if necessary.

Please see the product label for the expiry date of your product.

Cryopreservation:

1. Aspirate medium.
2. Wash with PBS and aspirate PBS.
3. Add Trypsin/EDTA (TE) solution to the cells and incubate at room temperature or 37°C for 5-10min, or until the cells detach.
4. Examine the cells under a microscope. When the cells start to detach, gently tap the side of the vessel to loosen the remaining cells.
5. Resuspend cells in 2% FBS in PBS and transfer to a 15ml conical bottom tube.
6. Centrifuge cells at 200×g for 4min.
7. Aspirate supernatant and gently resuspend cell pellet in Freezing medium (approx. 1Mio. cells/ml).
8. Transfer cell suspension into cryovial(s) and place them into a freezing container (“Mr.Frosty” or similar).
9. Place the freezing container at-80°C for 16-24h.
10. Transfer cryovials to liquid nitrogen vapor for long-term-storage.

Instructions for storage and cryopreservation

Material Safety Data Sheet (MSDS)