HEK293-CTLA4 is a recombinant clonal stable HEK293 cell line expressing full length human Cytotoxic T-lymphocyte protein 4 (CTLA-4). Surface expression of CTLA-4 was confirmed by flow cytometry using specific antibodies. Detailed information and characterisation can be found in the Product Sheet.
Target Aliases
CTLA4, CD152, GSE
Target Expression Level(s) Available
Medium-low (ca. 4000 molecules/cell as analysed by Quantibrite)
Cell Background
HEK293 (Human Embryonic Kidney), epithelial-like cells, adherent
Buying and Ordering the Cells
For non-profit organizations, cells are available for perpetual academic research under the Limited Research Use License.
For-profit entities can purchase cells for perpetual commercial use under the Limited Commercial Use License.
To request a quote, please email us at or use the contact form provided below.
We are already registered on several digital order processing platforms, if you would like to add us as a registered supplier, please contact us at and we are happy to help.
Supplied as
Each license includes 1 vial of >2Mio viable cells. Additional or replacement vials can be purchased for a fee of 450€.
Target Info
Background
CTLA4, also known as CD152, is an integral member of the immunoglobulin superfamily and functions as a key immune checkpoint protein. Predominantly found on T-cells, it regulates immune responses by competing with the stimulatory receptor CD28 for binding to B7 molecules (CD80 and CD86) on antigen-presenting cells.
Its higher affinity compared to CD28 leads to the downregulation of T cell activation, playing a vital role in preventing autoimmunity and maintaining self-tolerance.
Genetic variations in the CTLA4 gene are linked to various autoimmune diseases, highlighting its crucial role in immune balance.
In drug discovery and cell-based assays, CTLA4 is significant for its application in cancer immune checkpoint blockade therapy. Drugs like ipilimumab target CTLA4 to enhance anti-tumor immunity by activating T cells.
Studying CTLA4 in cell-based assays is essential for understanding immune regulation and developing new treatments for immune-related diseases, including cancer.
Field of Research
- Cancer progression
- Immune checkpoint
- Tumor microenvironment
Therapeutics and Binding Partners
CTLA4 (aka CD152) and CD28 both have the same binding partnersm but CTL4A binds with higher affinity and avidity.
Selected Therapeutics
- Ipilimumab
- Tremelimumab
Binding Partners
- CD80
- CD86
Product Details
Stable recombinant HEK293 cells at low and high confluency, cultured in HEK293 Growth Medium A (INS-ME-1041) as a 2D monolayer.
Expression of CTLA4 in HEK293-CTLA4 cells (INS-SF-1014) analyzed by Flow Cytometry.
Primary antibody: anti human CD152 (CTLA-4)-PE labelled, (Biolegend, Cat.No.: 369603) was used at 1:100 dilution as per manufacturer’s recommendations.
Flow Cytometry was performed according to the protocol provided under the „Protocols“ tab.
Quantification: approx. 4000 molecules per cell (medium-low) analyzed by BD QuantiBrite quantification kit (cat.no. 340495) according to the protocol provided under the „Protocols“ tab.
We recommend the following reagents for the cell culture of this cell line:
Growth Medium: HEK293 Growth Medium A (INS-ME-1041)
Freezing Medium: HEK293 Freezing Medium A (INS-SU-1025)
Coating: Collagen Coating Solution (INS-SU-1018), required ONLY for initial seeding after thawing
Please note that compositions for the above listed reagents are available on their respective product sites, as well as in the cell line Product Sheets, should you wish to prepare your own reagents.
- Product Sheet including all relevant protocols and instructions (PDF)
- Material Safety Data Sheet (PDF)
- Request CoA (Email)
Coming Soon!
Stay tuned for comprehensive protocols for this cell line, from routine cell cultures to assay manuals.
Coming Soon!
Coming Soon!
Material:
- PBS/EDTA solution
- 2% FBS/FCS in PBS (FACS Buffer)
- primary antibody (concentration is indicated under „Target expression“ tab)
Protocol:
Wash Protocol: Add FACS Buffer, resuspend cells gently, then centrifuge at 300×g for 5min.
- Prepare detection reagents in FACS buffer using the concentrations indicated under Materials.
- Aspirate medium from cells.
- Add PBS/EDTA solution to the cells and incubate at room temperature or 37°C for 5-10min, or until the cells detach.
- Wash cells 1×.
- Add primary antibody in FACS buffer, resuspend cells gently.
- Incubate at ambient temperature for 20-30min.
- Wash cells 2×.
- Resuspend in 100-200µl FACS Buffer.
- Analyse cells using a flow cytometer.
For a more detailed protocol, please see the BD Biosciences Quantibrite instructions.
Important: All instrument settings for fluorescence and compensation must be the same as the cellular assay settings.
- Reconstitute the BD Quantibrite beads using 0.5 mL of buffer, such as phosphate buffered saline (PBS) with sodium azide plus 0.5% bovine serum albumin (BSA), and vortex.
- Measure fluorescence of singlet beads using a flow cytometer and extract the geometric means of all four fluorescence peaks.
- Measure cells using the same instrument settings.
- Use the lot-specific values for the PE molecules per bead and linear regression to calculate the PE molecules per cell (approximation of target molecules per cell)
- For an even more meaningful result, contact the antibody provider for information on the number of fluorescent molecules per antibody.
Related Products
Product FAQ
Yes, for selected targets, we provide cell lines that exhibit varying expression levels. Detailed information regarding the availability of different expression levels can be found in both the ‚Product Summary‘ and ‚Product Details‘ sections.
Cell lines with available expression level variations are denoted by an abbreviation following the catalog number. For instance, ‚INS-SF-1020-MH‘ denotes a cell line with medium-high expression levels, whereas ‚INS-SF-1020-L‘ indicates a cell line with low expression of the same target.
Should you require a specific expression level not readily available in our standard product range, we also offer custom cell line generation services utilizing our advanced Landing Pad technology.
For several targets, we already have ready-to-use cell lines available in both CHO and HEK293 backgrounds. Please refer to Related Products section, or have a look at our product catalog to see if your target and the desired cell line are listed.
For any other specific cell line background such as A549, MIA PaCa-2, or any other cell line, we currently do not offer pre-established cell lines. However, we are pleased to provide a custom cell line development service. Through this service, we can express your target in virtually any cell background utilizing random integration techniques, tailored to meet your research needs.
For the best performance of this cell line, we advise using our Medium and Coating Solutions specified in the product sheet. These have been specially optimized for these cells.
For our off-the-shelf stable recombinant cell line, we also disclose the Medium composition in the Product Sheet so you can make your ownMedium.
All our stable recombinant cell lines are provided as adherent cultures, if not indicated otherwise in the Product Sheet.
Some of our CHO cell lines can be easily adapted to suspension culture. If a particular cell line can be cultured in suspension is indicated in the product summary section at the top of the page under Cell Background, as well as in the Product Sheet, where you will also find detailed protocols for suspension culture.
Our stable recombinant cell lines are generated using our proprietary Landing Pad Technology, in which we use targeted integration at pre-validated genomic locations to express the target. You can find more details on our Landing Pad technologies under our Landing Pad Services.
After cell line generation, target expression levels were validated by Flow Cytometry using the reagents and protocols indicated in the section Product Details or in the Product Sheet.
This cell line is BSL 1 according to German legislation. However, persons working with such cell lines and their employers are required to familiarize themselves with the national regulations and safety precautions, as they may differ. Our provided biosafety classification does not provide any exemption from this responsibility.
Literature and Reference
We love to hear about your research! Please let us know if you have published using our cells.