This freezing medium is optimized for the cryopreservation of our stable recombinant HEK293 cells (Type A). This freezing medium is supplied pre-mixed and ready-to-use.
Characteristics
- Ready-to-use
- Optimized for high viability
- Contains serum
Contents
- 30ml Freezing Medium ready-to-use
Cell Lines
- HEK293 cells (Type-A)
Composition
The complete formulation is available in the Product Details and the FAQ below.
Buying and Ordering
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Product Details
HEK293 Freezing Medium A is a specially developed cryopreservation medium for freezing of our HEK293 cells (Type-A).
This Medium is provided as a ready-to-use product.
The complete formulation of this freezing reagent is available:
- 70% (v/v) HEK293 Growth Medium A (INS-ME-1041) without Geneticin G418 Sulfat
- 20% (v/v) FBS/FCS
- 10% (v/v) DMSO
Store the products as follows if not indicated otherwise on the product label.
Freezing Medium -18 to -20°C, avoid repeated freeze-thaw cycles. Aliquot if necessary.
Please see the product label for the expiry date of your product.
Cryopreservation:
- Aspirate medium.
- Wash with PBS and aspirate PBS.
- Add Trypsin/EDTA (TE) solution to the cells and incubate at room temperature or 37°C for 5-10min, or until the cells detach.
- Examine the cells under a microscope. When the cells start to detach, gently tap the side of the vessel to loosen the remaining cells.
- Resuspend cells in 2% FBS in PBS and transfer to a 15ml conical bottom tube.
- Centrifuge cells at 200×g for 4min.
- Aspirate supernatant and gently resuspend cell pellet in Freezing medium (approx. 1Mio. cells/ml).
- Transfer cell suspension into cryovial(s) and place them into a freezing container (“Mr.Frosty” or similar).
- Place the freezing container at-80°C for 16-24h.
- Transfer cryovials to liquid nitrogen vapor for long-term-storage.
Instructions for storage and cryopreservation -coming soon!
Material Safety Data Sheet (MSDS) – coming soon!
Related Products
Product FAQ
Yes, this Medium is supplied ready-to-use and can be used as is for cryopreservation.
Yes, this Medium does contain FBS.
The complete formulation of this freezing reagent is available:
- 70% (v/v) HEK293 Growth Medium A (INS-ME-1041) without Geneticin G418 Sulfat
- 20% (v/v) FBS/FCS
- 10% (v/v) DMSO