Proprietary mix of extracellular matrix (ECM) proteins that is optimized for the culture of our immortalized alveolar cells and comes as a ready-to-use solution.
Characteristics
- Ready-to-use solution
- Blend of ECM proteins
- Coating solution
Contents
- 50, 100 or 300ml ready-to-use solution
Cell Lines
- CI-hAELVi (INS-CI-1015)
- CI-huArlo (INS-CI-1031)
Buying and Ordering
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Related Products
Product Details
huAEC Coating Solution is a specially developed coating solution for optimal attachment and growth of immortalized alveoalar cells CI-hAELVi (INS-CI-1015) and CI-huArlo (INS-CI-1031).
huAEC Coating Solution is provided as a ready-to-use solution.
Alveolar epithelial cells play a crucial role in the respiratory system by lining the alveoli, tiny air sacs within the lungs where gas exchange occurs. Our alveolar cells have been extensively used to model the air-blood-barrier in vitro.
Store the products as follows if not indicated otherwise on the product label.
Coating Solution: at 4 to 8°C
Coated cell culture vessels: sealed at 4 to 8°C
Please see the product label for the expiry date of your product.
To prepare coated cell culture surfaces:
- Cover the cell culture vessel with huAEC Coating (see info below for recommended volumes). Rock back and forth to cover entire surface if necessary.
- Incubate for at least 2h at 37°C in the incubator or overnight at 2–8°C.
- Aspirate huAEC Coating.
- Wash once with at least 4 volumes of sterile PBS.
- Aspirate PBS.
Use immediately or store coated cell culture plastic according to instructions provided under „Storage“.
Flask/Plate | Coating Volume
T75 | 3ml
T25 | 1ml
6-well | 0.7ml
12-well | 0.25ml
24-well | 0.1ml
48-well | 75µl
96-well | 50µl
Instructions for storage and coating
Material Safety Data Sheet (MSDS)
Product FAQ
Yes, this reagent is provided as a sterile, ready-to-use solution.
This is a proprietary blend of extracellular matrix proteins optimized for cell attachment and growth. We do not disclose the exact composition of this Coating Solution. However, if you have specific components that might interfere with any of your assays, we are happy to help you. Just reach out to us!
Depending on the product, we may be able to offer custom formulations, for example excluding specific supplements from the medium. Please contact us and we will work with you to find the best solution.