This low-serum medium is optimized for the differentiation of our immortalized osteoblasts. This medium is supplied pre-mixed and ready-to-use. The differentiation medium slows down proliferation and induces differentiation including mineralization.
For maintenance and expansion of our CI-huOB cells, please use our huOB Maintenance Medium (INS-ME-1006).
Characteristics
- Ready-to-use
- Low serum
- Differentiation medium
Contents
- 100ml Medium ready-to-use
Cell Lines
- CI-huOB (INS-CI-1005)
Buying and Ordering
To request a quote, please email us at or use the contact form provided below.
We are already registered on several digital order processing platforms, if you would like to add us as a registered supplier, please contact us at and we are happy to help.
Related Products
Product Details
huOB Medium is a specially developed osteoblast growth medium for the in vitro differentiation of our immortalized osteoblasts CI-huOB (INS-CI-1005).
It is designed for use in an incubator at 37° C with a 5% CO2 atmosphere.
This Medium is used to induce differentiation and mineralization of our CI-huOB osteoblasts. For routine maintenance and expansion please use our huOB Maintenance Medium (INS-ME-1006).
This Medium is provided as a ready-to-use product.
Osteoblasts are specialized cells primarily responsible for bone formation and bone mineralization. They produce and secrete the organic components of the bone matrix, including collagen, which provides the framework for deposition of calcium and other minerals, the so-called mineralization.
Store the products as follows if not indicated otherwise on the product label.
Basal Medium at 4 to 8°C
Supplement Mix at -18 to -20°C
Complete Medium at 4 to 8°C
Please see the product label for the expiry date of your product.
Prepare the complete Medium before use:
- Thaw Supplement Mix at ambient temperature.
- Add 30ml Supplement Mix to 500ml Basal Medium to obtain the complete Medium.
To prepare smaller volumes of complete Medium, aliquot Supplements and store at -20°C. For example, aliquot 5×6ml and then add 6ml Supplements to 100ml Basal Medium.
Instructions for storage and preparation
Material Safety Data Sheet (MSDS)
Product FAQ
Yes, this Medium is supplied ready-to-use and can be used as is for differentiation of CI-huOB.
Yes, this Medium does contain >5% FBS.
We do not disclose the exact composition of this Medium. However, if you have specific components that might interfere with any of your assays, we are happy to help you. Just reach out to us!
No, none of our standard media for our immortalized cells contains prophylactic antibiotics. You can supplement the medium with any standard cell culture antibiotic if you wish.
Depending on the product, we may be able to offer custom formulations, for example excluding specific supplements from the medium. Please contact us and we will work with you to find the best solution.