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CI-muINTEPI

Immortalized Murine Intestinal Epithelial Cells

CI-muINTEPI are immortalized murine fetal intestinal epithelial cells. Intestinal epithelial cells form the single layer of epithelium that lines both, the small and large intestine. They play vital roles in digestion, nutrient absorption, and barrier function within the digestive system. Three main cell types make up the intestinal epithelium, the absorptive epithelial cells (enterocytes), goblet cells and Paneth cells.

Characteristics

  • Microvilli formation
  • Expression of intestinal epithelial markers including ZO-1, E-cadherin, Connexin-43
  • Barrier and tight junction formation on cell culture inserts

muADINTESTI or muINTEPI?

We offer two different murine intestinal cell lines muADINTESTI (INS-CI-1018)and muINTEPI. We generally recommend using muADINTESTI cells, muINTEPI is a legacy product and only available upon request. See the FAQ for more information.

Buying and Ordering the Cells

For non-profit organizations, cells are available for perpetual academic research under the Limited Research Use License. To request a quote, please email us at or use the contact form provided below.

For-profit entities can purchase cells for commercial use starting with an Evaluation Agreement for preliminary testing. This is followed by custom, non-exclusive licensing agreements tailored to your needs. For further details, reach out to us at or via the contact form below.

Supplied as

Each license includes 1 vial of >0.5Mio viable cells. Additional or replacement vials can be purchased for a fee of 450€.

Catalog Number
INS-CI-1007
Features and Applications
  • Barrier formation
  • Tight junctions
  • Responsive to inflammatory stimuli
Price Non-Profit (Academic Research)
900€
Price For-Profit (Commercial Research)
Evaluation: 3500€ (3 Months) / 5000€ (6 Months)
Limited Research Use License

Product Details

Legacy Product!

This is a legacy product and is no longer supported by us. Please have a look at the FAQ below or get in touch with us.

Legacy Product!

This is a legacy product and is no longer supported by us. Please have a look at the FAQ below or get in touch with us.

We recommend the following reagents for the cell culture of this cell line:

Growth Medium: muINTEPI Medium (INS-ME-1023)

Coating: Collagen Coating Solution (INS-SU-1017)

Freezing Medium: Freezing Medium A (INS-SU-1004)

Please note that while you may be able to adapt the cells to different cell culture reagents, we can only guarantee optimal performance using the recommended reagents.

  • Product Sheet including all relevant protocols and instructions (PDF)
  • Material Safety Data Sheet (PDF)
  • Request CoA (Email)

Legacy Product!

This is a legacy product and is no longer supported by us. Please have a look at the FAQ below or get in touch with us.

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Medium kit for culture of immortalized intestinal epithelial cells.

  • Intestinal epithelial culture
  • Medium kit
  • 500ml
More details
and prices ->

Coating Solution for culture of various immortalized cells.

  • Various cell types
  • Coating solution
  • 50, 100 or 300ml
More details
and prices ->

Medium kit for cryopreservation of immortalized cells.

  • Cryopreservation Medium
  • Ready-to-use
  • 30ml
More details
and prices ->

Immortalized murine intestinal epithelial cells isolated from an adult mouse.

  • Barrier formation
  • Tight junctions
  • Organoid-like 3D culture
More details
and prices ->

Product FAQ

CI-muINTEPI and CI-muADINTESTI are both murine intestinal epithelial cell lines developed and distributed by us, but they originate from different source tissue, stages of tissue development and have distinct characteristics.

CI-muINTEPI cells were generated from murine fetal small intestinal tissue (Schwerk et al., 2013). They express typical epithelial markers and show polarized growth. CI-muINTEPI cells have been used in a wide range of infection and immunity studies, e.g., interferon signalling, Salmonella infection and Cryptosporidium infection.

CI-muADINTESTI are immortalized cells derived from murine adult intestinal tissue. Isolated primary cells were cultured as intestinal organoids before immortalization to maintain optimal functionality. muADINTESTI express typical epithelial markers and grow polarized. They form a barrier of approx. 2000 to 4000 Ohm×cm2 (TEER) already on day 5 after seeding on membrane cell culture inserts.

When selecting a cell culture model, consider the type of assay and experiment you would like to perform. When setting up a new model system, we recommend using CI-muADINTESTI cells for most applications. Overall, we found them to be more robust and more functional than CI-muINTEPI cells. However, if you would like to build upon already published results, which were generated using CI-muINTEPI cells, consider using them instead. We are happy to support you anytime – please get in touch with us!

For the best performance of this cell line, we advise using our Medium and Coating Solutions specified in the product sheet. These have been specially optimized for these cells. While it may be possible to adapt the cells to a different medium, we cannot assure their optimal performance outside our recommended Medium and Coating Solution. To validate any new culturing conditions, we suggest conducting comparative tests with our recommended protocols to confirm the cells‘ expected behavior.

All our cell lines were immortalized using our custom library of immortalization genes. This library contains a combination of specifically selected immortalization genes encoded in lentiviruses. For a detailed description of our immortalization technology refer to Lipps et al. Nat comm vol. 9,1 994. 2018.

Firstly, it is worth mentioning that passage number is not always a reliable indicator of cell ageing due to its dependence on variables like seeding densities and split ratios. However, they can provide useful guidance in routine cell culture.

Our cell lines are truly immortalized, allowing for indefinite culturing. Nonetheless, we advise caution beyond passage 50. While more stable than typical cancer cell lines, our cells can still undergo changes with prolonged culture. We recommend periodically assessing cell performance after passage 50.

Typically, our immortalized cells are provided at passages 5 to 15. To avoid running out of material, we suggest banking cells immediately after initial expansion. This strategy ensures you always have sufficient cells for your research.

Should there be any concerns about depleting your cell stock, you have the option to order a new vial of cells as a replacement for a handling fee.

This cell line is BSL 1 according to German legislation. However, persons working with such cell lines and their employers are required to familiarize themselves with the national regulations and safety precautions, as they may differ. Our provided biosafety classification does not provide any exemption from this responsibility. 

Yes, you can use our immortalized cell lines for commercial purposes with the appropriate license. We provide an initial evaluation phase for our cell lines at a flat fee of €3,500 for a 3-month license, or €5,000 for a 6-month license. During this period, use of the cells is limited to internal research, development, and validation.

For commercial use beyond the evaluation phase, we offer various licensing options, such as:

  • Internal research and development
  • CRO license (providing research services to third parties)
  • Assay license

 

Please contact us to find the licensing option that best fits your needs.

Our immortalized cell lines are fully characterized and can be expanded indefinitely, making them a one-time purchase for perpetual use. This differs from primary cells, which are consumables you would need to repurchase. The licensing model not only respects the perpetual use value but also allows us to adjust fees based on your specific use case. Consider two scenarios: Customer A wants the cells for a single assay at one site for a limited time, while Customer B plans to use them globally across multiple sites for drug discovery and additionally transfer them to a CRO. Charging both customers the same one-time fee would not account for the vastly different use cases. That’s why our license fees are tailored to fit your unique needs.

Literature and Reference

Schwerk, Johannes et al. “Generation of mouse small intestinal epithelial cell lines that allow the analysis of specific innate immune functions.” PloS one vol. 8,8 e72700. 5 Aug. 2013, doi:10.1371/journal.pone.0072700

Kuźmińska-Bajor, Marta et al. “Type 1 fimbriae are important factors limiting the dissemination and colonization of mice by Salmonella Enteritidis and contribute to the induction of intestinal inflammation during Salmonella invasion.” Frontiers in microbiology vol. 6 276. 9 Apr. 2015, doi:10.3389/fmicb.2015.00276

Kesselring, Rebecca et al. “IRAK-M Expression in Tumor Cells Supports Colorectal Cancer Progression through Reduction of Antimicrobial Defense and Stabilization of STAT3.” Cancer cell vol. 29,5 (2016): 684-696. doi:10.1016/j.ccell.2016.03.014

Bhushal, Sudeep et al. “Cell Polarization and Epigenetic Status Shape the Heterogeneous Response to Type III Interferons in Intestinal Epithelial Cells.” Frontiers in immunology vol. 8 671. 12 Jun. 2017, doi:10.3389/fimmu.2017.00671

Grzymajlo, Krzysztof et al. “The Novel Type 1 Fimbriae FimH Receptor Calreticulin Plays a Role in Salmonella Host Specificity.” Frontiers in cellular and infection microbiology vol. 7 326. 19 Jul. 2017, doi:10.3389/fcimb.2017.00326

Sünderhauf, Annika et al. “Regulation of epithelial cell expreszed C3 in the intestine – Relevance for the pathophysiology of inflammatory bowel disease?.” Molecular immunology vol. 90 (2017): 227-238. doi:10.1016/j.molimm.2017.08.003

Selvakumar, Tharini A et al. “Identification of a Predominantly Interferon-λ-Induced Transcriptional Profile in Murine Intestinal Epithelial Cells.” Frontiers in immunology vol. 8 1302. 16 Oct. 2017, doi:10.3389/fimmu.2017.01302

Li, Min et al. “Induction of a Long Noncoding RNA Transcript, NR_045064, Promotes Defense Gene Transcription and Facilitates Intestinal Epithelial Cell Responses against Cryptosporidium Infection.” Journal of immunology (Baltimore, Md. : 1950) vol. 201,12 (2018): 3630-3640. doi:10.4049/jimmunol.1800566

Forero, Adriana et al. “Differential Activation of the Transcription Factor IRF1 Underlies the Distinct Immune Responses Elicited by Type I and Type III Interferons.” Immunity vol. 51,3 (2019): 451-464.e6. doi:10.1016/j.immuni.2019.07.007

Büssow, Konrad et al. “ER intrabody-mediated inhibition of interferon α secretion by mouse macrophages and dendritic cells.” PloS one vol. 14,4 e0215062. 16 Apr. 2019, doi:10.1371/journal.pone.0215062

El Abbas, Sophie et al. “Epithelial RABGEF1 deficiency promotes intestinal inflammation by dysregulating intrinsic MYD88-dependent innate signaling.” Mucosal immunology vol. 13,1 (2020): 96-109. doi:10.1038/s41385-019-0211-z

He, Wei et al. “Cryptosporidial Infection Suppresses Intestinal Epithelial Cell MAPK Signaling Impairing Host Anti-Parasitic Defense.” Microorganisms vol. 9,1 151. 12 Jan. 2021, doi:10.3390/microorganisms9010151

 

We love to hear about your research! Please let us know if you have published using our cells.