This serum-free medium is optimized for the culture of our immortalized fibroblasts CI-huFIB. The medium kit contains Basal Medium and a Supplement Mix, which need to be combined to obtain the complete medium.
Characteristics
- Medium kit
- Serum-free
- Requires Collagen coating
- Expansion medium
Contents
- 500ml Basal Medium
- 30ml Supplement mix
Cell Lines
- CI-huFIB (INS-CI-1010)
Buying and Ordering
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Product Details
Serum-free huFIB Medium is a specially developed fibroblast growth medium for the serum-free in vitro culture of our immortalized fibroblasts CI-huFIB (INS-CI-1010).
Please note that serum-free culture of our CI-huFIBs requires Collagen-coated cell culture surfaces (Collagen Coating Solution; INS-SU-1017).
It is designed for use in an incubator at 37° C with a 5% CO2 atmosphere.
This Medium is provided as a kit consisting of Basal Medium and Supplement Mix in separate sterile packing, which need to be combined to obtain the complete, ready-to-use medium.
Fibroblasts are the essential cells of the connective tissue, where they produce extracellular matrix, contribute to tissue repair and wound healing, and maintain tissue structure. Fibroblasts are often used together with epithelial cells in co-culture models to model tissue fibrosis.
Store the products as follows if not indicated otherwise on the product label.
Basal Medium at 4 to 8°C
Supplement Mix at -18 to -20°C
Complete Medium at 4 to 8°C
Please see the product label for the expiry date of your product.
Prepare the complete Medium before use:
- Thaw Supplement Mix at ambient temperature.
- Add 30ml Supplement Mix to 500ml Basal Medium to obtain the complete Medium.
To prepare smaller volumes of complete Medium, aliquot Supplements and store at -20°C. For example, aliquot 5×6ml and then add 6ml Supplements to 100ml Basal Medium.
Instructions for storage and preparation
Material Safety Data Sheet (MSDS)
Product FAQ
Our cell culture media kits consist of 500ml Basal Medium and 30ml Supplement Mix. Both components need to be combined to obtain the complete, ready-to-use medium. Detailed protocols for reagent preparation and storage can be found in the Instructions
No, this Medium does not contain serum.
We do not disclose the exact composition of this Medium. However, if you have specific components that might interfere with any of your assays, we are happy to help you. Just reach out to us!
No, none of our standard media for our immortalized cells contains prophylactic antibiotics. You can supplement the medium with any standard cell culture antibiotic if you wish.
No, although we usually ship the Supplement Mix frozen and we recommend storage at -18 to -20°C , it may arrive thawed from transport. This does not affect the quality of the product. However, please avoid repeated freeze-thaw cycles. Aliquot the Supplement Mix
Depending on the product, we may be able to offer custom formulations, for example excluding specific supplements from the medium. Please contact us and we will work with you to find the best solution.