Lung Epithelial Cells

The investigation of the human lung and its diseases is an important issue for drug development processes, although it is hampered by the availability of suitable in vitro models. Using our CI-SCREEN technology (recently published in Nature Communications; Lipps et al., 2018) we developed a novel immortal human lung epithelial cell line displaying a primary like phenotype.

Specifications of CI-huAEC

Characteristic expression of epithelial cell markers (e.g. E-Cadherin, ZO-1)
Maintained polarity
Tight barrier formation
Low permeability

Following assays have been performed and validated with the CI-huAEC cell line:

Transepithelial Electrical Resistance (TEER)-formation
Rhodamine permeation
GPCR profiling (cAMP regulation/Ca2+ regulation)
TGF-beta stimulation

Product Name	Cat. No.	Information	Price
CI-huAEC Human Airway Epithelial cells	INS-CI-1011	Instruction Manual	Get prices

Related Products

Product Name	Cat. No.	Size	Price
huAEC Medium (ready-to-use)	INS-ME-1013-100ml	100 ml	€ 75.00
huAEC Medium (ready-to-use)	INS-ME-1013-500ml	500 ml	€ 290.00
huAEC Coating solution	INS-SU-1018-20ml	20 ml	€ 45.00
huAEC Coating solution	INS-SU-1018-100ml	100 ml	€ 170.00
Freezing medium	INS-SU-1004	30 ml	€ 35.00

Related Protocols for Cultivation of CI-huAEC

Instruction Manual (including protocols)

Literature

Nanoparticle induced barrier function assessment at liquid–liquid and air–liquid interface in novel human lung epithelia cell lines. Leibrock L et al., Toxicol. Res., 2019, 8, 1016-1027. doi: 10.1039/C9TX00179D. (2019) [link].
Investigation on Ciliary Functionality of Different Airway Epithelial Cell Lines in 3D Cell Culture. Lodes NT et al., Tissue Eng Part A 2019 Nov 7. doi: 10.1089/ten.TEA.2019.0188. (2019) [link].

Functionally Immortalized