Functionally Immortalized

[Legacy Product] huAEC Lung Epithelial Cells

Legacy Product

CI-huAEC have been replaced with CI-huBroBEC, an improved cell line with a better lung epithelial phenotype. We still make replacement vials and reagents availabale for legacy huAEC user, but recommend using CI-huBroBEC when setting up a new lung epithelial cell culture system. Please get in touch if you have any questions.

The investigation of the human lung and its diseases is an important issue for drug development processes, although it is hampered by the availability of suitable in vitro models. Using our CI-SCREEN technology (recently published in Nature Communications; Lipps et al., 2018) we developed a novel immortal human lung epithelial cell line displaying a primary like phenotype.

Specifications of CI-huAEC

  • Characteristic expression of epithelial cell markers (e.g. E-Cadherin, ZO-1)
  • Maintained polarity
  • Tight barrier formation
  • Low permeability

Following assays have been performed and validated with the CI-huAEC cell line:

  • Transepithelial Electrical Resistance (TEER)-formation
  • Rhodamine permeation
  • GPCR profiling (cAMP regulation/Ca2+ regulation)
  • TGF-beta stimulation
Product NameCat. No.InformationPrice
[Legacy Product] CI-huAEC
Human Airway Epithelial cells
[Legacy Product] INS-CI-1011Instruction ManualGet prices

Related Products

Product NameCat. No.SizePrice
huAEC Medium (ready-to-use)INS-ME-1013-100ml100 ml€ 82.00
huAEC Medium (ready-to-use)INS-ME-1013-500ml500 ml€ 315.00
huAEC Coating solutionINS-SU-1018-20ml20 ml€ 49.00
huAEC Coating solution INS-SU-1018-100ml100 ml€ 185.00
Freezing mediumINS-SU-100430 ml€ 39.00

Related Protocols for Cultivation of CI-huAEC


  • Nanoparticle induced barrier function assessment at liquid–liquid and air–liquid interface in novel human lung epithelia cell lines. Leibrock L et al., Toxicol. Res., 2019, 8, 1016-1027. doi: 10.1039/C9TX00179D. (2019) [link].
  • Investigation on Ciliary Functionality of Different Airway Epithelial Cell Lines in 3D Cell Culture. Lodes NT et al., Tissue Eng Part A 2019 Nov 7. doi: 10.1089/ten.TEA.2019.0188. (2019) [link].

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