Stable cell lines for drug discovery using random integration
Benefits of generating stable cell lines with us
Any cell background
We can work with any commercially available cell lines, or with any cell line from your lab.
Variety of delivery systems
We offer a number of different viral and non-viral gene delivery systems to ensure that we generate a stable cell line, even for otherwise hard-to-transfect or hard-to-transduce cell lines.
One-stop-shop for stable cells
From sourcing the parental cells, to generating the cells to producing cell banks – we can take care of everything for your. One single point of contact only.
Highly customized
License free
No royalties, no license fees, no hidden costs. We only charge for the service we provide.
Random integration technology
Features and Applications
-> Drug discovery
Fully characterized cell lines for drug discovery. From simple overexpression of surface protein to complex high-throughput-screening capable cell systems.
-> Reporter cell lines
We can generate reporter cell lines suitable for any type of assay using fluorescence, luminescence or colorimetric readouts.
-> Tumor target cells
Expression of cancer targets in your target cell line for ADCC, CDC and ADCP assays. Combine with reporter technology for a complete bioassay.
-> Inducible expression
Inducible expression of your target for ON/OFF switching or titration of the target expression level, ideal for toxic targets or precise control of protein expression levels.
Landing pad stable cells project workflow
-> We provide you with an honest feasibility assessment
Based on your project idea, we draft a project proposal including:
- a feasibility assessment
- suggested project structure and milestones
- timeline and cost estimates
Thanks to our extensive experience generating thousands of stable cell lines, we will tell you if your project is a low-, mid-, or high-risk project.
-> We design and organize the gene synthesis
We design the ideal expression construct for your target – all we need is a target name, Gene ID or amino acid sequence. Together with you, we work on improving and modifying the sequence. For example. We may suggest adding a leader sequences or tags to improve protein expression and detection.
We then initiate, monitor and organize the gene synthesis together with our trusted partners.
This usually takes between 1 and 3 weeks depending on the sequence length and complexity.
-> Rapid cloning into our optimized in-house vectors
We design all synthesized genes ready for integration into our molecular biology workflow minimizing the time required for cloning.
We clone your target into our in-house plasmids or in lentiviral backbones.
Molecular cloning usually takes between 1 and 2 weeks. If your project requires lentiviral gene transfer, lentivirus production will add between 2-3 weeks to project timelines.
-> We have established efficient transfection and transduction protocols for a variety of cell lines
We use our optimized transfection or transduction protocols to deliver your target with high efficiency.
We can use antibiotic selection markers or fluorescent markers to identify and select positive cells.
Transfection and expansion usually takes between 2-4 weeks depending on the cell type.
-> We make sure the cell line fits your requirements
We analyze target expression levels and provide additional characterization as necessary. Our default assays are: flow cytometry, qPCR or ELISA. In addition we offer a range of different assays to characterize the cell line.
Here is a non-exhaustive list of characterisation methods we offer in house or via subcontractors:
DNA/RNA level
- RT-qPCR
- NGS
- RNASeq
Protein level
- IF/ICC
- ELISA
- Flow cytometry
- WB
Functional assays
- second messenger assays (calcium, cAMP etc.) by fluorescence or luminescence
- live cell imaging (fluorescence or brightfield) for short- or long-term assays (Cellcyte X, Zeiss CD7)
- barrier formation via TEER
- plate reader-based assays
- Electrophysiology
-> We make sure everything looks good before we ship the cells
We perform extensive quality control to ensure you receive the best possible cell line. We offer a range of QC options, some of which are included as standard in our projects, others are optional:
- contaminations (bacteria, mycoplasma, fungi)
- human pathogens
- STR profiling, species identification
- cell numbers and viability
- karyotyping
As part of our QC, we can also prepare cell banks ranging from research cell banks (<50Mio cells) up to small master cell banks (up to 1000Mio cells).
We provide all protocols and results generated within the project to you in a comprehensive written report.
Pricing
-> Pricing for stable cell line development projects using random integration can vary significantly across projects
-> We are happy to provide you with a preliminary proposal and cost estimate based on your project idea
Stable cells using random integration Frequently Asked Questions (FAQ)
How are the cells generated?
To generate cell lines in any cell line we use traditional random integration technology, with the option to generate either polyclonal pools or monoclonal cell lines.
We us a range of standard gene delivery systems; other technologies are available upon request.
Non-viral:
- Lipofection (various systems)
- Electroporation/Nucleofection
- Calcium phosphate
Viral:
- Lentivirus
Can I use your stable cells for commercial purposes?
Generally, most commercial research applications are allowed and you receive a royalty-free license to our IP to use the cells for your research with completion of the service project.
Any other commercial uses may be possible as well (offering research services, release assays, incorporation into your products) but they may require separate licenses.
What is the BSL of the stable cell lines?
If the parental cells are BSL1, the stable cell lines will be classified BSL1 as well.
What are the lead times for your stable cell services?
We do not have any lead times and can start your project immediately.
How do I order your cell services?
The first step is to get in touch with us and tell us about your project idea. We will then provide you with a quote, which you can use for generating a purchase order.
You can then submit this PO to and we will start the project.
If we need to be registered as a supplier in your database, please contact .
In addition, we are listed on multiple digital purchasing platforms.
What is the cost of a stable cell line generation project?
Cost for stable cell line generation can vary significantly across different projects. Here are two example projects and estimated costs for each of them:
Polyclonal overexpression in standard cell line
Overexpression of a low-risk target in a standard cell line with established transfection protocols. Detection of the target by flow cytometry using a commercially available antibody. Generation of polyclonal pools only and banking of 15 Mio cells.
Estimated costs: 3 000–4 000€
Monoclonal reporter cell line in a non-standard cell line
Design of a reporter construct. Optimization of transfection protocols. Integration and validation of the reporter construct using 3 reference compounds. Assay establishment and optimization. Generation of dose-response curves as part of the cell line characterization. Generation of monoclonal cells and banking of a 150 Mio cell research cell bank.
Estimated costs: 15 000–25 000€
Please note that these examples are provided for reference only and pricing of your project may vary.
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