Functionally Immortalized

huVEC (hu)

The endothelium is the thin layer of cells that lines the interior surface of blood vessels and lymphatic vessels, forming an interface between circulating blood or lymph in the lumen and the rest of the vessel wall. InSCREENeX currently offers human endothelial cells isolated from the vein of the human umbilical cord (HUVEC). This cell line has been extensively characterized in our recently published Nature Communications paper [Lipps et al., 2018].

Specifications of CI-huVEC

  • Typical surface marker expression (CD31, Tie1, Tie2, CD309)
  • Von Willebrand factor expression

Following assays have been performed and validated with the CI-huVEC cell line:

  • Tube formation matrigel
  • Acetylated LDL uptake
  • eNos activity
  • in vitro angiogenesis (Heiss et al., 2015).
  • VEGF induced proliferation
  • TNF-alpha stimulation
  • Barrier formation
Product NameCat. No.InformationPrice
Human umbilical vein endothelial cells
INS-CI-1002Instruction ManualGet prices

Related Products

Product NameCat. No.SizePrice
huVEC Medium (ready-to-use)INS-ME-1011500 ml€ 195.00
Gelatin solution 2%INS-SU-1015-50ml50 ml€ 35.00
Gelatin solution 2%INS-SU-1015-300ml300 ml€ 162.00
Freezing mediumINS-SU-100430 ml€ 41.00

Related Protocols for Cultivation of CI-huVEC


  • Endothelial cell spheroids as a versatile tool to study angiogenesis in vitro. Heiss et al. 2015, The FASEB Journal [link]
  • Expansion of functional personalized cells with specific transgene combinations. Lipps et al. 2018, Nature Communications [link]
  • Biallelic CCM3 mutations cause a clonogenic survival advantage and endothelial cell stiffening. Schwefel et al. 2019, J Cell Mol Med [link]

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