Intestinal Epithelial Cells
Epithelial tissues line the cavities and surfaces of structures throughout the body, and also form many glands. Functions of epithelial cells include secretion, selective absorption, protection, transcellular transport and detection of sensation. InSCREENeX currently offers epithelial cells isolated from the murine intestine. The cells have been characterized in detail as "IEC" cells in Schwerk et al., 2018.
muINTEPI or muADINTESTI cells?
We offer two murine intestinal epithelial cells, muINTEPI (INS-CI-1007) and muADINTESTI (INS-CI-1018), providing a range of options to best suit your experiments and assays.
muINTEPI cells were generated from murine fetal small intestinal tissue (Schwerk et al., 2013). They express typical epithelial markers and show polarized growth. muINTEPI cells have been used in a wide range of infection and immunity studies, e.g., interferon signalling, Salmonella infection and Cryptosporidium infection.
muADINTESTI are immortalized cells derived from murine adult intestinal tissue. Isolated primary cells were cultured as intestinal organoids before immortalization to maintain optimal functionality. muADINTESTI express typical epithelial markers and grow polarized. They form a barrier of approx. 2000 to 4000 Ohm×cm2 (TEER) already on day 5 after seeding on membrane cell culture inserts.
When selecting a cell culture model, consider the type of assay and experiment you would like to perform. When setting up a new model system, we recommend using muADINTESTI cells for most applications. Overall, we found them to be more robust and more functional than muINTEPI cells. However, if you would like to build upon already published results, which were generated using muINTEPI cells, consider using them instead. We are happy to support you anytime – please get in touch with us!
|Product Name||Cat. No.||Information||Price|
Murine intestinal epithelial cells
|INS-CI-1007||Instruction Manual||Get prices|
|Product Name||Cat. No.||Size||Price|
|muINTEPI Medium (ready-to-use)||INS-ME-1005||500 ml||€ 155.00|
|Collagen solution||INS-SU-1017-50ml||50 ml||€ 30.00|
|Collagen solution||INS-SU-1017-300ml||300 ml||€ 140.00|
|Freezing medium||INS-SU-1004||30 ml||€ 35.00|
Related Protocols for Cultivation of muINTEPI
- Induction of a Long Noncoding RNA Transcript, NR_045064, Promotes Defense Gene Transcription and Facilitates Intestinal Epithelial Cell Responses against Cryptosporidium Infection. Li et al., J. Immunol. 16 November 2018 [link]
- Identification of a Predominantly Interferon-λ-Induced Transcriptional Profile in Murine Intestinal Epithelial Cells. Selvakumar et al., Front. Immunol., 16 October 2017 [link]
- Regulation of epithelial cell expressed C3 in the intestine – Relevance for the pathophysiology of inflammatory bowel disease? Sünderhauf et al., Molecular Immunology Volume 90, October 2017, Pages 227-238 [link]
- The Novel Type 1 Fimbriae FimH Receptor Calreticulin Plays a Role in Salmonella Host Specificity. Grzymajlo et al., Front. Cell. Infect. Microbiol., 19 July 2017 [link]
- Cell Polarization and Epigenetic Status Shape the Heterogeneous Response to Type III Interferons in Intestinal Epithelial Cells. Bhushal et al., Front. Immunol., 12 June 201 [link]
- IRAK-M Expression in Tumor Cells Supports Colorectal Cancer Progression through Reduction of Antimicrobial Defense and Stabilization of STAT3. Kesselring et al. Cancer Cell Volume 29, Issue 5, 9 May 2016, Pages 684-696 [link]
- Type 1 fimbriae are important factors limiting the dissemination and colonization of mice by Salmonella Enteritidis and contribute to the induction of intestinal inflammation during Salmonella invasion. Kuzminska-Bajor et al., 2015, Front. Microbiol. [link]
- Generation of Mouse Small Intestinal Epithelial Cell Lines That Allow the Analysis of Specific Innate Immune Functions. Schwerk et al., 2013, PLOS ONE [link]